RASA2/3 Polyklonaler Antikörper

RASA2/3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27835-1-AP

Synonyme

GAP1(IP4BP), GAP1IP4BP, GAPIII, Ins P4 binding protein, RAS p21 protein activator 3, RASA2/3, RASA3

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Galerie der Validierungsdaten

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  WB analysis of 3T3-L1 using 27835-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27835-1-AP (RASA2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27835-1-AP (RASA2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 3T3-L1-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27835-1-AP bindet in WB, ELISA RASA2/3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASA2/3 fusion protein Ag27152
• Vollständiger Name: RAS p21 protein activator 3
• Berechnetes Molekulargewicht: 834 aa, 96 kDa
• Beobachtetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: BC038456
• Gene symbol: RASA3
• Gene ID (NCBI): 22821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rasa3 is a GTPase activating protein of the GAP1 family which targets R-Ras and Rap1. While R-Ras has been extensively studied due to its involvement in cancer, Rap1 has recently attracted a lot of attention due to its central role in development and morphogenesis of higher organisms, especially in the cardiovasculature. This antibody can react with RASA2 and RASA3.

Publikationen

Species	Application	Title
human	WB	Arterioscler Thromb Vasc Biol; Lipid Receptor GPR31 (G-Protein-Coupled Receptor 31) Regulates Platelet Reactivity and Thrombosis Without Affecting Hemostasis.; Authors - Layla Van Doren
mouse	WB	Front Immunol; The Rac-GEF Tiam1 controls integrin-dependent neutrophil responses; Authors - Kirsti Hornigold