RAP1A Monoklonaler Antikörper

RAP1A Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1G12D8

Rezensionen (1)

Kat-Nr. : 68125-1-Ig

Synonyme

C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21

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Galerie der Validierungsdaten

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  WB analysis using 68125-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68125-1-Ig (RAP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human liver cancer using 68125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MOLT-4-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68125-1-Ig bindet in WB, IHC, ELISA RAP1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAP1A fusion protein Ag9463
• Vollständiger Name: RAP1A, member of RAS oncogene family
• Berechnetes Molekulargewicht: 184 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014086
• Gene symbol: RAP1A
• Gene ID (NCBI): 5906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

Publikationen

Species	Application	Title
mouse	WB	Autoimmunity; RGS7 silence protects palmitic acid-induced pancreatic β-cell injury by inactivating the chemokine signaling pathway; Authors - Yurong Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed between 50-150 kDa were observed . Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ARPE-19

RAP1A Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:68125-1-Ig)