MOK Polyklonaler Antikörper

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• KD/KO Validated

MOK Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23926-1-AP

Synonyme

MOK, MOK protein kinase, RAGE, RAGE 1, RAGE1, renal tumor antigen, Renal tumor antigen 1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 23926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23926-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 23926-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 23926-1-AP

  1X10^6 HeLa cells were stained with 0.2ug MOK antibody (23926-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

23926-1-AP bindet in WB, IHC, IF, FC, ELISA MOK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MOK fusion protein Ag20849
• Vollständiger Name: renal tumor antigen
• Berechnetes Molekulargewicht: 231 aa, 27 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 85 kDa
• GenBank-Zugangsnummer: BC053536
• Gene symbol: RAGE
• Gene ID (NCBI): 5891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities; Authors - Jing Hang ; KD Validated
	WB	J Biol Chem; Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.; Authors - Ran Li