RAF1 Polyklonaler Antikörper

RAF1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 51140-1-AP

Synonyme

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

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Galerie der Validierungsdaten

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  IHC staining of human prostate cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 51140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51140-1-AP (RAF1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

51140-1-AP bindet in IHC, ELISA RAF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Berechnetes Molekulargewicht: 648 aa, 73 kDa
• GenBank-Zugangsnummer: BC018119
• Gene symbol: RAF1
• Gene ID (NCBI): 5894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Publikationen

Species	Application	Title
human		Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse		J Neuroinflammation; Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage.; Authors - Xiaowei Fei
human		Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human		Biochem Pharmacol; 1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.; Authors - Yuhang Zhou
mouse		Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
human		Food Funct; Dandelion root extract affects ESCC progression via regulating multiple signal pathways.; Authors - Xiaofang Duan