RAF1 Polyklonaler Antikörper

RAF1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 26863-1-AP

Synonyme

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

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Galerie der Validierungsdaten

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  WB analysis of DU 145 using 26863-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26863-1-AP (RAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

26863-1-AP bindet in WB, IHC, IF, ELISA RAF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAF1 fusion protein Ag25423
• Vollständiger Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Berechnetes Molekulargewicht: 648 aa, 73 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC018119
• Gene symbol: RAF1
• Gene ID (NCBI): 5894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Publikationen

Species	Application	Title
human	WB	Oncogene; Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.; Authors - Feifei Zhang
human	WB	Virol Sin; PLX8394, a RAF inhibitor, inhibits enterovirus 71 replication by blocking RAF/MEK/ERK signaling; Authors - Chengyuan Wu
human	WB	Mol Cell Probes; Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway; Authors - Huiyuan Pang
human	WB	FEBS Open Bio; CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.; Authors - Dali Zhao
mouse	WB	Toxins (Basel); Subchronic Microcystin-LR Aggravates Colorectal Inflammatory Response and Barrier Disruption via Raf/ERK Signaling Pathway in Obese Mice; Authors - Yue Yang
human	WB,IF	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang