RAD50 Polyklonaler Antikörper

RAD50 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 29390-1-AP

Synonyme

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

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Galerie der Validierungsdaten

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  WB analysis using 29390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29390-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 29390-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29390-1-AP bindet in WB, IHC, IF, ELISA RAD50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD50 fusion protein Ag29949
• Vollständiger Name: RAD50 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 154 kDa
• GenBank-Zugangsnummer: NM_005732
• Gene symbol: RAD50
• Gene ID (NCBI): 10111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Veronica (Verified Customer) (11-14-2022)

The antibody seems to work correctly. However, further tests need to be done.

• Applications: Immunofluorescence
• Cell Tissue Type: hTERT RPE-1