RAD23A Polyklonaler Antikörper

RAD23A Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 51033-1-AP

Synonyme

HHR23A, HR23A, RAD23A

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 51033-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 51033-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 51033-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 51033-1-AP

  IP Result of anti-RAD23A (IP:51033-1-AP, 4ug; Detection:51033-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human lymphoma using 51033-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 51033-1-AP (RAD23A antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Jurkat-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

51033-1-AP bindet in WB, IP, IHC, IF, ELISA RAD23A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAD23A fusion protein Ag0716
• Vollständiger Name: RAD23 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC014026
• Gene symbol: RAD23A
• Gene ID (NCBI): 5886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome. This antibody is a rabbit polyclonal antibody raised against a fusion protein of full length human RAD23A.

Publikationen

Species	Application	Title
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
monkey	WB, IF	PLoS One; Valosin-containing protein (VCP/p97) is an activator of wild-type ataxin-3.; Authors - Laço Mário N MN
mouse	WB	Cell Rep; Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in mice; Authors - Lei Xing