• Featured Product
  • KD/KO Validated

RAC1/2/3 Polyklonaler Antikörper

RAC1/2/3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16117-1-AP

Synonyme

p21 Rac3, RAC1/2/3, RAC3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSH-SY5Y-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

KD/KOSee 3 publications below
WBSee 8 publications below

Produktinformation

16117-1-AP bindet in WB, IP, ELISA RAC1/2/3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RAC1/2/3 fusion protein Ag9045
Vollständiger Name ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
Berechnetes Molekulargewicht 192 aa, 21 kDa
Beobachtetes Molekulargewicht 21 kDa
GenBank-ZugangsnummerBC015197
Gene symbol RAC3
Gene ID (NCBI) 5881
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.

Protokolle

Produktspezifische Protokolle
WB protocol for RAC1/2/3 antibody 16117-1-APProtokoll herunterladen
IP protocol for RAC1/2/3 antibody 16117-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cancer

F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.

Authors - Su Dong
mouseWB

Front Cell Neurosci

A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.

Authors - Sira A Franchi
  • KO Validated
humanWB

Mol Biol Cell

Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.

Authors - Brian J Rosenberg
  • KD Validated
humanWB

BMC Cancer

Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.

Authors - Gest Caroline C
mouseWB

Glycoconj J

Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide.

Authors - Erika Di Biase
humanWB

Asian Pac J Cancer Prev

Silencing of rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.

Authors - Tie-Qin Liu
  • KD Validated