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RAC1/2/3 Polyklonaler Antikörper
RAC1/2/3 Polyklonal Antikörper für IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16117-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | SH-SY5Y-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe |
Erfolgreiche IP | Maushirngewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 8 publications below |
Produktinformation
16117-1-AP bindet in WB, IP, ELISA RAC1/2/3 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | RAC1/2/3 fusion protein Ag9045 |
Vollständiger Name | ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) |
Berechnetes Molekulargewicht | 192 aa, 21 kDa |
Beobachtetes Molekulargewicht | 21 kDa |
GenBank-Zugangsnummer | BC015197 |
Gene symbol | RAC3 |
Gene ID (NCBI) | 5881 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for RAC1/2/3 antibody 16117-1-AP | Protokoll herunterladen |
IP protocol for RAC1/2/3 antibody 16117-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Mol Cancer F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation. | ||
Front Cell Neurosci A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.
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Mol Biol Cell Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.
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BMC Cancer Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines. | ||
Glycoconj J Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide. | ||
Asian Pac J Cancer Prev Silencing of rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.
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