RABEPK/p40 Polyklonaler Antikörper

RABEPK/p40 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15105-1-AP

Synonyme

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 15105-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15105-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15105-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15105-1-AP

  IP result of anti-RABEPK/p40 (IP:15105-1-AP, 4ug; Detection:15105-1-AP 1:1000) with HeLa cells lysate 2080 ug.

• 

  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15105-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RABEPK/p40 antibody (15105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15105-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RABEPK/p40 (15105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15105-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RABEPK/p40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RABEPK/p40 fusion protein Ag2593
• Vollständiger Name: Rab9 effector protein with kelch motifs
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC065725
• Gene symbol: RABEPK
• Gene ID (NCBI): 10244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.