RABEP2 Monoklonaler Antikörper

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• KD/KO Validated

RABEP2 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1A4A4

Kat-Nr. : 60125-1-Ig

Synonyme

FRA, Rabaptin 5beta, RABEP2, RABPT5B

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 60125-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 60125-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60125-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60125-1-Ig bindet in WB, IHC,ELISA RABEP2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RABEP2 fusion protein Ag6197
• Vollständiger Name: rabaptin, RAB GTPase binding effector protein 2
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC058900
• Gene symbol: RABEP2
• Gene ID (NCBI): 79874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.