RAB5A Monoklonaler Antikörper

RAB5A Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1B6A5

Kat-Nr. : 66339-1-Ig

Synonyme

RAB5, RAB5A, Ras related protein Rab 5A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66339-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66339-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66339-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66339-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66339-1-Ig(RAB5A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

66339-1-Ig bindet in WB, IHC, IF, ELISA RAB5A und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB5A fusion protein Ag16781
• Vollständiger Name: RAB5A, member RAS oncogene family
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC001267
• Gene symbol: RAB5A
• Gene ID (NCBI): 5868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.

Publikationen

Species	Application	Title
human	IF	Bioact Mater; Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.; Authors - Jin Wang
human	IF	Clin Ther; Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019.; Authors - Yoel Benarroch
human	WB	J Exp Clin Cancer Res; TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells.; Authors - Ruizhi He
		Commun Biol; Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.; Authors - Kun Meng