RAB3GAP2 Polyklonaler Antikörper

RAB3GAP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 24599-1-AP

Synonyme

KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

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  WB analysis using 24599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24599-1-AP (RAB3GAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24599-1-AP bindet in WB, IHC, ELISA RAB3GAP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3GAP2 fusion protein Ag20254
• Vollständiger Name: RAB3 GTPase activating protein subunit 2 (non-catalytic)
• Berechnetes Molekulargewicht: 1393 aa, 156 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC146760
• Gene symbol: RAB3GAP2
• Gene ID (NCBI): 25782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

BING (Verified Customer) (08-04-2021)

A reliable Ab to probe RAB3GAP2 with the right MW of 150 kd.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse brain and postmortembrain lysates

RAB3GAP2 Antibody Western Blot validation (1:5000 dilution) in mouse brain and postmortembrain lysates (Cat no:24599-1-AP)