RAB3B/C Polyklonaler Antikörper

RAB3B/C Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15774-1-AP

Synonyme

RAB3B, Ras related protein Rab 3B

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Galerie der Validierungsdaten

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  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 15774-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15774-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15774-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15774-1-AP

  Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15774-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug RAB3B antibody (15774-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

15774-1-AP bindet in WB, IHC, IF, FC, ELISA RAB3B/C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3B/C fusion protein Ag8441
• Vollständiger Name: RAB3B, member RAS oncogene family
• Berechnetes Molekulargewicht: 219 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC005035
• Gene symbol: RAB3B
• Gene ID (NCBI): 5865
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Ann Neurol; PMID: 32167609; Authors - Jimena Monzón-Sandoval
mouse	WB	Hum Mol Genet; Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.; Authors - Gaynor A Smith
human	WB,IF	Cell Death Dis; Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B; Authors - Jinxiu Hu