RAB31-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB31-specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 16182-1-AP

Synonyme

Rab22B, RAB31, RAB31-specific, Ras related protein Rab 22B, Ras related protein Rab 31

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 16182-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16182-1-AP (RAB31-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16182-1-AP (RAB31-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16182-1-AP (RAB31-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16182-1-AP (RAB31-specific Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16182-1-AP bindet in WB, IHC, IF, CoIP, ELISA RAB31-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB31, member RAS oncogene family
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_006868
• Gene symbol: RAB31
• Gene ID (NCBI): 11031
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.

Publikationen

Species	Application	Title
human	WB,IF	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei ; KD Validated
human	WB	Int J Biol Sci; Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.; Authors - Yujie Huang ; KD Validated
human	WB,IHC,CoIP	Front Oncol; RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.; Authors - Chao-Tao Tang ; KD Validated
human	WB	PLoS One; Human liver cell trafficking mutants: characterization and whole exome sequencing.; Authors - Fei Yuan
human,mouse	WB,IHC,IF	Int J Biochem Cell Biol; Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.; Authors - Chan Qiu ; KD Validated
human	WB,IHC	Life Sci; Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.; Authors - Li Yang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Showed up reasonably well using 12 ug protein lysate with fluorescent secondary

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: K562, KCL22 and THP-1