RAB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15420-1-AP

Synonyme

RAB2, RAB2A, Ras related protein Rab 2A

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 15420-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15420-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15420-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15420-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15420-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15420-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15420-1-AP (RAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15420-1-AP (RAB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15420-1-AP (RAB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15420-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15420-1-AP (RAB2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HeLa-Zellen, Mauslungengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

15420-1-AP bindet in WB, IHC, IF, ELISA RAB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB2 fusion protein Ag7708
• Vollständiger Name: RAB2A, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC008929
• Gene symbol: RAB2A
• Gene ID (NCBI): 5862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize both RAB2A and RAB2B.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Cell Host Microbe; A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.; Authors - Cheryl N Miller ; KD Validated
human	WB	Nat Commun; Arsenic targets Pin1 and cooperates with retinoic acid to inhibit cancer-driving pathways and tumor-initiating cells.; Authors - Shingo Kozono
		Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
		EMBO Rep; RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs.; Authors - Hiroaki Kajiho
human	WB, IF	Cell Rep; The Rab2A GTPase promotes breast cancer stem cells and tumorigenesis via Erk signaling activation.; Authors - Man-Li Luo ; KD Validated