RAB27B Monoklonaler Antikörper

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• KD/KO Validated

RAB27B Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2B9G9

Kat-Nr. : 66944-1-Ig

Synonyme

C25KG, RAB27B, Ras related protein Rab 27B

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66944-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66944-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66944-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 66944-1-Ig

  1x10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB27B (66944-1-Ig, Clone:2B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

66944-1-Ig bindet in WB, IHC, IF, FC, ELISA RAB27B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB27B fusion protein Ag18756
• Vollständiger Name: RAB27B, member RAS oncogene family
• Berechnetes Molekulargewicht: 218 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC027474
• Gene symbol: RAB27B
• Gene ID (NCBI): 5874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Publikationen

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB,IF	J Biol Chem; The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of α-synuclein.; Authors - Rachel Underwood ; KD Validated
mouse	IF	FEBS Open Bio; Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells.; Authors - Tomoko Kadowaki
human	WB	Am J Reprod Immunol; Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis; Authors - Jing Shan