RAB27A-specific Polyklonaler Antikörper

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• KD/KO Validated

RAB27A-specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 16868-1-AP

Synonyme

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAB27A-specific, RAM, Ras related protein Rab 27A

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Galerie der Validierungsdaten

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  WB analysis using 16868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K562 using 16868-1-AP

  WB result of RAB27A antibody (16868-1-AP, 1:1000) with si-Control and si-RAB27A transfected K562 cells.

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  WB analysis of HL-60 using 16868-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16868-1-AP (RAB27A-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16868-1-AP (RAB27A-specific antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, K562, PC-12-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16868-1-AP bindet in WB, IHC, ELISA RAB27A-specific und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB27A, member RAS oncogene family
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_004580
• Gene symbol: RAB27A
• Gene ID (NCBI): 5873
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Publikationen

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Involvement of Rab11 in osteoblastic differentiation: Its up-regulation during the differentiation and by tensile stress; Authors - Lay Thant