RAB26 Polyklonaler Antikörper

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• KD/KO Validated

RAB26 Polyklonal Antikörper für FC (Intra), IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14284-1-AP

Synonyme

RAB26, Ras related protein Rab 26, V46133

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Galerie der Validierungsdaten

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  WB analysis of mouse pancreas using 14284-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14284-1-AP (RAB26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14284-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14284-1-AP (RAB26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 14284-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14284-1-AP (RAB26 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 14284-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14284-1-AP (RAB26 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of BxPC-3 using 14284-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 14284-1-AP (RAB26 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 14284-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RAB26 (14284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: BxPC-3-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

14284-1-AP bindet in WB, IP, IHC, IF, FC (Intra), ELISA RAB26 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB26 fusion protein Ag5581
• Vollständiger Name: RAB26, member RAS oncogene family
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC066913
• Gene symbol: RAB26
• Gene ID (NCBI): 25837
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Sci Total Environ; Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons; Authors - Zhuo Ma
human	WB,IF	J Cell Sci; RAB26 coordinates lysosome traffic and mitochondrial localization.; Authors - Ramon U Jin
human	IHC	Cell Death Dis; SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.; Authors - Nianli Liu ; KO Validated
human	WB, IHC	Cell Death Dis; Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.; Authors - Huiying Liu
human	WB	J Immunol Res; Novel Circulating Tumour Cell-Related Risk Model Indicates Prognosis and Immune Infiltration in Lung Adenocarcinoma.; Authors - Lu Liang ; KD Validated
human,mouse	WB,IHC	Free Radic Biol Med; ROS induced the Rab26 promoter hypermethylation to promote cigarette smoking-induced airway epithelial inflammation of COPD through activation of MAPK signaling; Authors - Bin-Feng He ; KD Validated