RAB14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB14 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15662-1-AP

Synonyme

FBP, RAB 14, RAB14, Ras related protein Rab 14

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 15662-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15662-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15662-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15662-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15662-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 15662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, humanes Hirngewebe, Mausnierengewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

15662-1-AP bindet in WB, IHC, IF, ELISA RAB14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB14 fusion protein Ag8092
• Vollständiger Name: RAB14, member RAS oncogene family
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC006081
• Gene symbol: RAB14
• Gene ID (NCBI): 51552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Physiol Biochem; Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer.; Authors - Bo Guo
human	IHC,WB	Onco Targets Ther; Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers.; Authors - Jun Zhang
human	WB	PLoS One; Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.; Authors - Bo Guo
human	WB,IHC	Virchows Arch; PMID: 30267303; Authors - Jinnian Ge ; KD Validated
human	WB,IHC	Tumour Biol; Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway.; Authors - Rui Hou ; KD Validated
human	WB	Eur Rev Med Pharmacol Sci; STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14.; Authors - C Du