RAB11FIP1 Polyklonaler Antikörper

RAB11FIP1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16778-1-AP

Synonyme

NOEL1A, Rab coupling protein, rab11 FIP1, RAB11FIP1, RCP

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16778-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16778-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 16778-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16778-1-AP

  IP Result of anti-RAB11FIP1 (IP:16778-1-AP, 4ug; Detection:16778-1-AP 1:600) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 16778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16778-1-AP

  Immunofluorescent analysis of HeLa cells using 16778-1-AP (RAB11FIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COS-7-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• RIP: See 2 publications below

Produktinformation

16778-1-AP bindet in WB, RIP, IP, IHC, ELISA RAB11FIP1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB11FIP1 fusion protein Ag10179
• Vollständiger Name: RAB11 family interacting protein 1 (class I)
• Berechnetes Molekulargewicht: 649aa,71 kDa; 1283aa,137 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC077720
• Gene symbol: RAB11FIP1
• Gene ID (NCBI): 80223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,RIP	Mol Cancer; CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein; Authors - Chi Hin Wong
human	WB	Brief Bioinform; mi-IsoNet: systems-scale microRNA landscape reveals rampant isoform-mediated gain of target interaction diversity and signaling specificity.; Authors - Li Guo
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
human	WB,IHC	Am J Cancer Res; In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer; Authors - Qiankun Zhu