RAB11A-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB11A-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 20229-1-AP

Synonyme

Rab 11, RAB11, RAB11A, RAB11A-Specific, Ras related protein Rab 11A, YL8

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Galerie der Validierungsdaten

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  WB analysis using 20229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20229-1-AP

  WB result of RAB11A-Specific antibody (20229-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A-Specific transfected HeLa cells.

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  WB analysis of mouse colon using 20229-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20229-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20229-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20229-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 20229-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 20229-1-AP

  IP Result of anti-RAB11A-Specific (IP:20229-1-AP, 3ug; Detection:20229-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 20229-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, Maus-Kolongewebe, Mauslungengewebe, MCF-7-Zellen, PC-12-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 8 publications below

Produktinformation

20229-1-AP bindet in WB, IP, IHC, IF, ELISA RAB11A-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB11A, member RAS oncogene family
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_004663
• Gene symbol: RAB11A
• Gene ID (NCBI): 8766
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Publikationen

Species	Application	Title
human	IF	J Thromb Haemost; Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII.; Authors - Jenny Chia
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC ; KD Validated
		J Cell Biol; BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.; Authors - William J Monis
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
mouse	WB	Cell Mol Gastroenterol Hepatol; Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis.; Authors - Olga A Mareninova
human	IF	Br J Pharmacol; An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.; Authors - Weisheng Lu