QDPR Polyklonaler Antikörper

QDPR Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 14908-1-AP

Synonyme

DHPR, Dihydropteridine reductase, HDHPR, PKU2, QDPR, SDR33C1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 14908-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14908-1-AP (QDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14908-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14908-1-AP (QDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14908-1-AP

  IP Result of anti-QDPR (IP:14908-1-AP, 4ug; Detection:14908-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human ovary tumor using 14908-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14908-1-AP (QDPR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14908-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14908-1-AP (QDPR antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Lebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

14908-1-AP bindet in WB, IP, IHC QDPR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: QDPR fusion protein Ag6705
• Vollständiger Name: quinoid dihydropteridine reductase
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC000576
• Gene symbol: QDPR
• Gene ID (NCBI): 5860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dihydropteridine reductase (QDPR), also named as DHPR and HDHPR, is an essential enzyme in the hydroxylating system of the aromatic amino acids, since it catalyses the regeneration of tetrahydrobiopterin (BH4), the natural cofactor of phenylalanine, tyrosine, and tryptophan hydroxylases, from the quininoid-dihydrobiopterin produced in these coupled reactions(PMID:8326489). The QDPR protein is active as a dimer, with a subunit Mr of 26 kDa(PMID:7627180). This protein belongs to the short-chain dehydrogenases/reductases (SDR) family. Defects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C)(PMID:11153907).

Publikationen

Species	Application	Title
mouse	WB	Mol Autism; RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.; Authors - Natasha L Pacheco
mouse	WB	Front Mol Neurosci; Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.; Authors - Kalina Wiatr
human	WB	Genes Genomics; Subproteomic profiling from renal cortices in OLETF rats reveals mutations of multiple novel genes in diabetic nephropathy.; Authors - Zhiguo Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hua (Verified Customer) (01-20-2023)

Very good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver and mouse brain

QDPR Antibody Western Blot validation (1:1000 dilution) in Mouse liver and mouse brain (Cat no:14908-1-AP)