Polyglutamine Monoklonaler Antikörper

Polyglutamine Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

Anwendung

WB

Konjugation

Unkonjugiert

CloneNo.

MW1

Kat-Nr. : 65239-1-Ig

Synonyme

Glutamine repeats, HTT, Huntington disease protein, Polyglutamine, polyQ

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Galerie der Validierungsdaten

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  WB analysis of Recombinant protein using 65239-1-Ig

  Western blot analysis of anti-polyglutamine antibody (MW1) binding to huntingtin exon 1 fusion proteins with variable numbers of glutamines. MW1 bound to huntingtin exon 1 proteins with normal and expanded polyQ repeats but did not bind the TRX tag control. (Owens, Gwen E et al. J Mol Biol. 2015 Jul 31;427(15):2507-2519.)

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65239-1-Ig bindet in WB Polyglutamine und zeigt Reaktivität mit

• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Polyglutamine
• GenBank-Zugangsnummer: N/A
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Huntington's disease is a neurodegenerative disorder caused by the expansion of a polyglutamine (polyQ) repeat in the N-terminal portion of huntingtin protein to a length above 35-40 units (PMID: 26047735; 19507258). The mutational expansion of polyglutamine above a critical length causes a toxic gain of function in huntingtin and results in neuronal death. In the course of the disease, expanded huntingtin is proteolyzed, becomes abnormally folded, and accumulates in oligomers, fibrils, and microscopic inclusions (PMID: 25336039). The anti-polyglutamine (polyQ) antibody MW1 specifically binds the polyQ domain of huntingtin exon 1. On western blot, the MW1 clone strongly prefers to bind to the expanded polyQ repeat form of Htt, displaying no detectable binding to normal huntingtin (PMID: 11719267).