Podocalyxin Polyklonaler Antikörper

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• KD/KO Validated

Podocalyxin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-18150

Synonyme

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

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Galerie der Validierungsdaten

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  IF Staining of human kidney using CL488-18150

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Podocalyxin antibody (CL488-18150) at dilution of 1:200.

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  IF Staining of human kidney using CL488-18150

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Podocalyxin antibody (CL488-18150) at dilution of 1:200.

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  FC experiment of HeLa using CL488-18150

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Podocalyxin (CL488-18150) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Nierengewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-18150 bindet in IF, FC (Intra) Podocalyxin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Podocalyxin fusion protein Ag12844
• Vollständiger Name: podocalyxin-like
• Berechnetes Molekulargewicht: 526 aa, 55 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC093730
• Gene symbol: PODXL
• Gene ID (NCBI): 5420
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C