Phospho-p130Cas / BCAR1 (Tyr410) Polyklonaler Antikörper

Phospho-p130Cas / BCAR1 (Tyr410) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30887-1-AP

Synonyme

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

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Galerie der Validierungsdaten

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  WB analysis using 30887-1-AP

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 30887-1-AP (Phospho-p130Cas / BCAR1 (Tyr410) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with p130Cas / BCAR1 antibody (16815-1-AP) subsequently.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: pervanadate treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30887-1-AP bindet in WB, ELISA Phospho-p130Cas / BCAR1 (Tyr410) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: breast cancer anti-estrogen resistance 1
• Berechnetes Molekulargewicht: 870 aa, 93 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC062556
• Gene symbol: BCAR1
• Gene ID (NCBI): 9564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

p130Cas, encoded by the breast cancer anti-estrogen resistance 1 (BCAR1) gene, is a scaffold protein that integrates large multi-protein complexes in response to stimuli such as hormones, growth factors, and integrin engagement. Upon environmental signals, Src family members and focal adhesion kinase (FAK) phosphorylate the p130Cas substrate domain (SD). This active p130Cas can recruit adaptor proteins such as Crk and Nck to its phosphorylated SD. (PMID: 34830244)