Phospho-mTOR (Ser2448) Rekombinanter Antikörper

Phospho-mTOR (Ser2448) Rekombinant Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3L18

Kat-Nr. : CL488-80596

Synonyme

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1

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Galerie der Validierungsdaten

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  IF Staining of HEK-293 using CL488-80596

  Immunofluorescent analysis of (-20°C Ethanol) fixed PMA treated HEK-293 cells using CoraLite® Plus 488 Phospho-mTOR (Ser2448) antibody (CL488-80596, Clone: 3L18 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-80596

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-mTOR (Ser2448) (CL488-80596, Clone:3L18) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: PMA treated HEK-293 cells
• Erfolgreiche Detektion in FC: Mit Calyculin A behandelte HeLa-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-80596 bindet in IF, FC (Intra) Phospho-mTOR (Ser2448) und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: FK506 binding protein 12-rapamycin associated protein 1
• Berechnetes Molekulargewicht: 289 kDa
• Beobachtetes Molekulargewicht: 250-289 kDa
• GenBank-Zugangsnummer: BC117166
• Gene symbol: FRAP1
• Gene ID (NCBI): 2475
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.