Phospho-YAP1 (Ser397) Polyklonaler Antikörper

Phospho-YAP1 (Ser397) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 29018-1-AP

Synonyme

65 kDa Yes associated protein, Phospho-YAP1 (Ser397), YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

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Galerie der Validierungsdaten

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  WB analysis using 29018-1-AP

  Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29018-1-AP (Phospho-YAP1 (Ser397) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Serum-free treated NIH/3T3 cells, mit 10%igem fetalen Rinderserum behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

29018-1-AP bindet in WB, IHC, ELISA Phospho-YAP1 (Ser397) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Yes-associated protein 1, 65kDa
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC038235
• Gene symbol: YAP1
• Gene ID (NCBI): 10413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

Publikationen

Species	Application	Title
human	WB	Front Immunol; The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective; Authors - Xueqing Hu
mouse	WB	Arch Biochem Biophys; Oridonin suppresses the growth of glioblastoma cells via inhibiting Hippo/YAP axis; Authors - Chen Wang
mouse,human	WB	J Nat Med; Ruscogenin ameliorates dasatinib-induced intestinal barrier dysfunction via ErbB4/YAP and ROCK/MLC pathways; Authors - Juan Huang
human	WB,IHC	Cell Death Dis; PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling; Authors - Hualin Chen
human	WB	Mol Carcinog; LATS-regulated nuclear-cytoplasmic translocation of SREBP2 inhibits hepatocellular carcinoma cell migration and invasion via epithelial-mesenchymal transition; Authors - Feng Zhang
human	WB	Biochem Biophys Res Commun; IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression; Authors - Yan Xu