Phospho-STK11/LKB1 (Thr189) Rekombinanter Antikörper

Phospho-STK11/LKB1 (Thr189) Rekombinant Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5J9

Kat-Nr. : 80127-1-RR

Synonyme

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

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Galerie der Validierungsdaten

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  WB analysis using 80127-1-RR

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 80127-1-RR

  Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 80127-1-RR

  Non-treated HEK-293 and Calyculin A treated HEK-293 cells subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of PC-3 using 80127-1-RR

  Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11/LKB1 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-3 using 80127-1-RR

  1X10^6 PC-3 cells untreated (dashed lines) or Calyculin A treated (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-STK11 (Thr189) (80127-1-RR, Clone:5J9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen, Mit Calyculin A behandelte PC-3-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: Mit Calyculin A behandelte PC-3-Zellen
• Erfolgreiche Detektion in FC: Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80127-1-RR bindet in WB, IF, FC, ELISA Phospho-STK11/LKB1 (Thr189) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: serine/threonine kinase 11
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC007981
• Gene symbol: STK11
• Gene ID (NCBI): 6794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.