Phospho-STK11/LKB1 (Thr189) Polyklonaler Antikörper

Phospho-STK11/LKB1 (Thr189) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 28965-1-AP

Synonyme

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

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Galerie der Validierungsdaten

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  WB analysis using 28965-1-AP

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

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  WB analysis using 28965-1-AP

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte PC-3-Zellen, Mit UV behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28965-1-AP bindet in WB, ELISA Phospho-STK11/LKB1 (Thr189) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: serine/threonine kinase 11
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC007981
• Gene symbol: STK11
• Gene ID (NCBI): 6794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071)

Publikationen

Species	Application	Title
human	WB	Cells; Psoralen Suppresses Lipid Deposition by Alleviating Insulin Resistance and Promoting Autophagy in Oleate-Induced L02 Cells.; Authors - Yuhao Wang