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Phospho-STAT5A (Tyr694) Rekombinanter Antikörper

Phospho-STAT5A (Tyr694) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2P21

Kat-Nr. : CL555-80115

Synonyme

MGF, STAT5, STAT5A

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of TF-1 cells using CoraLite®555-conjugated Phospho-STAT5A (Tyr694) Re (CL555-80115)

    FC experiment of TF-1 using CL555-80115

    1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.03 ug CoraLite®555 Anti-Human Phospho-STAT5A (Tyr694) (CL555-80115, Clone:2P21), or 0.03 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMit GM-CSF behandelte TF-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.03 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-80115 bindet in FC (Intra) Phospho-STAT5A (Tyr694) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name signal transducer and activator of transcription 5A
Berechnetes Molekulargewicht 794 aa, 92 kDa
Beobachtetes Molekulargewicht90-95 kDa
GenBank-ZugangsnummerBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.