Phospho-STAT5A(Ser726)/STAT5B (Ser731) Polyklonaler Antikörper

Phospho-STAT5A(Ser726)/STAT5B (Ser731) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 28951-1-AP

Synonyme

MGF, Phospho-STAT5 (Ser726), Phospho-STAT5A(Ser726)/STAT5B (Ser731), STAT5, STAT5A

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Galerie der Validierungsdaten

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  WB analysis using 28951-1-AP

  Non-treated TF-1 and GM-SCF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 28951-1-AP (Phospho-STAT5A(Ser726)/STAT5B (Ser731) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit GM-CSF behandelte TF-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28951-1-AP bindet in WB, ELISA Phospho-STAT5A(Ser726)/STAT5B (Ser731) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 5A
• Berechnetes Molekulargewicht: 794 aa, 92 kDa
• Beobachtetes Molekulargewicht: 90-95 kDa
• GenBank-Zugangsnummer: BC027036
• Gene symbol: STAT5A
• Gene ID (NCBI): 6776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 50% Glyzerin, pH 7,3 und 0,05% BSA.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. This antibody can recognize the phosphorylation of STAT5A at Ser726 and the phosphorylation of STAT5B at Ser731.

Publikationen

Species	Application	Title
human	WB	Endocrinology; A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration; Authors - Ruonan Li
human	WB	PeerJ; RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA; Authors - Jin Fan