Phospho-RPS6KA1 (Thr359/Ser363) Polyklonaler Antikörper

Phospho-RPS6KA1 (Thr359/Ser363) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 29225-1-AP

Synonyme

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

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Galerie der Validierungsdaten

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  WB analysis using 29225-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

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  WB analysis using 29225-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit λ-Phosphatase behandelte NIH/3T3-Zellen, Mit PDGF behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29225-1-AP bindet in WB, ELISA Phospho-RPS6KA1 (Thr359/Ser363) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Berechnetes Molekulargewicht: 735 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83-90 kDa
• GenBank-Zugangsnummer: BC014966
• Gene symbol: RPS6KA1
• Gene ID (NCBI): 6195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,02% Natriumazid und 50% Glyzerin, pH 7,3 und 0,05% BSA.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

Publikationen

Species	Application	Title
pig	WB	JTCVS Open; Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium; Authors - Dwight D Harris