Phospho-RIPK1 (Ser161) Monoklonaler Antikörper

Phospho-RIPK1 (Ser161) Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2G1

Kat-Nr. : CL488-66854

Synonyme

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161) , Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

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Galerie der Validierungsdaten

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  IF Staining of U2OS using CL488-66854

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 Phospho-RIPK1 (Ser161) antibody (CL488-66854, Clone: 1B2G1 ) at dilution of 1:100.

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  FC experiment of HEK-293T using CL488-66854

  1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-RIPK1 (Ser161) (CL488-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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  FC experiment of PC-3 using CL488-66854

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite488 Anti-Human Phospho-RIPK1 (Ser161) (CL488-66854, Clone:1B2G1), or 0.13 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: U2OS-Zellen
• Erfolgreiche Detektion in FC: Mit Calyculin A behandelte PC-3-Zellen, mit Calyculin A behandelte HEK-293T-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66854 bindet in IF, FC (Intra) Phospho-RIPK1 (Ser161) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 74 kDa, 45 kDa
• GenBank-Zugangsnummer: NM_003804
• Gene symbol: RIPK1
• Gene ID (NCBI): 8737
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).