Phospho-PRKD1 (Ser916) Polyklonaler Antikörper

Phospho-PRKD1 (Ser916) Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 28928-1-AP

Synonyme

Phospho-PRKD1 (Ser916), PKC MU, PKCM, PKD, PKD1, PRKCM, PRKD1, Protein kinase C mu type, Protein kinase D, protein kinase D1

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Galerie der Validierungsdaten

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  WB analysis using 28928-1-AP

  Non-treated NIH/3T3 and Insulin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28928-1-AP (Phospho-PRKD1 (Ser916) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Insulin behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28928-1-AP bindet in WB, ELISA Phospho-PRKD1 (Ser916) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein kinase D1
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_001330069
• Gene symbol: PRKD1
• Gene ID (NCBI): 5587
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 50% Glyzerin, pH 7,3 und 0,05% BSA.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)

Publikationen

Species	Application	Title
rat	WB	Food Chem Toxicol; G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.; Authors - Yao Liu
rat	WB	Biochem Biophys Res Commun; Protein kinase D1 promotes the survival of random-pattern skin flaps in rats; Authors - Jianpeng Chen