Phospho-PERK/EIF2AK3 (Ser719) Polyklonaler Antikörper

Phospho-PERK/EIF2AK3 (Ser719) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 29546-1-AP

Synonyme

DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, Phospho-PERK/EIF2AK3 (Ser719), WRS

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Galerie der Validierungsdaten

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  WB analysis using 29546-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29546-1-AP

  Non-treated HeLa cells and Thapsigargin treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit λ-Phosphatase behandelte HeLa-Zellen, Thapsigargin treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

29546-1-AP bindet in WB, ELISA Phospho-PERK/EIF2AK3 (Ser719) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Berechnetes Molekulargewicht: 1116 aa, 125 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC126354
• Gene symbol: EIF2AK3
• Gene ID (NCBI): 9451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Publikationen

Species	Application	Title
human	WB	Mar Drugs; Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma; Authors - Yun Huang
human	WB	World J Gastroenterol; Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress; Authors - Xiao-Xue Hou
human	WB	Tissue Cell; XBP1s gene of endoplasmic reticulum stress enhances proliferation and osteogenesis of human periodontal ligament cells; Authors - Ziwei Cui
	WB	Fish Shellfish Immunol; Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp; Authors - Jie Cui
human	WB	Cell Death Dis; PDIA4 confers resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell carcinoma; Authors - Lichun Kang
rat	WB	Ecotoxicol Environ Saf; The ZIP9-centered androgen pathway compensates for the 2605 MHz radiofrequency electromagnetic radiation-mediated reduction in resistance to H2O2 damage in Sertoli cells of adult rats; Authors - Gang Yu