Phospho-P62,SQSTM1 (Ser349) Polyklonaler Antikörper

Phospho-P62,SQSTM1 (Ser349) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 29503-1-AP

Synonyme

P62, Phospho-p62/SQSTM1 (Ser349), sequestosome 1, SQSTM1, Ubiquitin binding protein p62

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Galerie der Validierungsdaten

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  WB analysis using 29503-1-AP

  Non-treated HeLa cells and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62,SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 29503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62,SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MG132 treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

29503-1-AP bindet in WB, ELISA Phospho-P62,SQSTM1 (Ser349) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sequestosome 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC017222
• Gene symbol: SQSTM1
• Gene ID (NCBI): 8878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Channel HCN4 mutation R666Q associated with sporadic arrhythmia decreases channel electrophysiological function and increases protein degradation; Authors - Hongrui Wang
human	WB	J Virol; The Porcine Cyclic GMP-AMP Synthase-STING Pathway Exerts an Unusual Antiviral Function Independent of Interferon and Autophagy; Authors - Sen Jiang
human	WB	Drug Des Devel Ther; α-Mangostin Inhibited M1 Polarization of Macrophages/Monocytes in Antigen-Induced Arthritis Mice by Up-Regulating Silent Information Regulator 1 and Peroxisome Proliferators-Activated Receptor γ Simultaneously; Authors - Yi-Jin Wu