Phospho-P53 (Ser46) Polyklonaler Antikörper

Phospho-P53 (Ser46) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 28960-1-AP

Synonyme

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser46), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Galerie der Validierungsdaten

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  WB analysis using 28960-1-AP

  Non-treated HT-29, phosphatase inhibitor treated and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28960-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28960-1-AP

  Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28959-1-AP (Phospho-P53 (Ser46) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, mit λ-Phosphatase behandelte HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

28960-1-AP bindet in WB, ELISA Phospho-P53 (Ser46) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: TP53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .

Publikationen

Species	Application	Title
human	WB	Apoptosis; High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.; Authors - Chieh-Hsin Chen
human	WB	Exp Cell Res; RPS14 promotes the development and progression of glioma via p53 signaling pathway; Authors - Shangwei Hu
human	WB	Mol Cell Probes; Long noncoding RNA RMRP ameliorates doxorubicin-induced apoptosis by interacting with PFN1 in a P53-Dependent manner; Authors - Juexing Li
human	WB	Tissue Cell; DEPDC1B enhances malignant phenotypes of multiple myeloma through upregulating CCNB1 and inhibiting p53 signaling pathway; Authors - Hairong Fei