Phospho-P53 (Ser392) Polyklonaler Antikörper
Phospho-P53 (Ser392) Polyklonal Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 28963-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | λ phosphatase treated HEK-293 cells |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 1 publications below |
Produktinformation
28963-1-AP bindet in WB, ELISA Phospho-P53 (Ser392) und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | tumor protein p53 |
Berechnetes Molekulargewicht | 44 kDa |
Beobachtetes Molekulargewicht | 53 kDa |
GenBank-Zugangsnummer | BC003596 |
Gene symbol | TP53 |
Gene ID (NCBI) | 7157 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.(PMID: 19935675, PMID: 24379683)
Protokolle
Produktspezifische Protokolle | |
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WB protocol for Phospho-P53 (Ser392) antibody 28963-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Chem Biol Interact Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65 |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Andria (Verified Customer) (01-29-2023) | This antibody detects 2 bands. I believe the correct one is the lower because it is induced upon actinomycin treatment
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