Phospho-GRB10 (Ser476) Polyklonaler Antikörper

Phospho-GRB10 (Ser476) Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28873-1-AP

Synonyme

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, Phospho-GRB10 (Ser476), RSS

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Galerie der Validierungsdaten

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  WB analysis using 28873-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28873-1-AP (Phospho-GRB10 (Ser476) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit λ-Phosphatase behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28873-1-AP bindet in WB,ELISA Phospho-GRB10 (Ser476) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: growth factor receptor-bound protein 10
• Berechnetes Molekulargewicht: 536aa,61 kDa; 594aa,67 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC024285
• Gene symbol: GRB10
• Gene ID (NCBI): 2887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)