Phospho-EIF4B (Ser422) Rekombinanter Antikörper

Phospho-EIF4B (Ser422) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1K3

Kat-Nr. : 82550-1-RR

Synonyme

EIF 4B, EIF4B, Phospho-EIF4B (Ser422), PRO1843

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Galerie der Validierungsdaten

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  WB analysis using 82550-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82550-1-RR (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82550-1-RR bindet in WB, ELISA Phospho-EIF4B (Ser422) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 4B
• Berechnetes Molekulargewicht: 611 aa, 69 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC073154
• Gene symbol: EIF4B
• Gene ID (NCBI): 1975
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other EIF proteins are EIF4A, EIF4F. EIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of EIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). Ser422 is regarded as the major modification site of EIF4B that is phosphorylated in response to amino-acid refeeding(PMID: 22750809).