Phospho-EIF2S1 (Ser51) Monoklonaler Antikörper

Phospho-EIF2S1 (Ser51) Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1A4A11

Kat-Nr. : 68023-1-Ig

Synonyme

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51)

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Galerie der Validierungsdaten

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  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (HRP-66009) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis using 68023-1-Ig

  Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control.

• 

  WB analysis of HEK-293 using 68023-1-Ig

  Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of PC-3 using 68023-1-Ig

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-EIF2S1 (Ser51) (68023-1-Ig, Clone:1A4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, Mit Calyculin A behandelte HeLa-Zellen, mit Calyculin A behandelte HSC-T6-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen, Mit Calyculin A behandelte PC-3-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68023-1-Ig bindet in WB, FC, ELISA Phospho-EIF2S1 (Ser51) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_004094
• Gene symbol: EIF2S1
• Gene ID (NCBI): 1965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Mitosis exit followed by death in interphase prevents the development of polyploid giant cancer cells; Authors - Juan Jesus Vicente