Periostin Polyklonaler Antikörper

Periostin Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27794-1-AP

Synonyme

OSF 2, OSF2, Osteoblast specific factor 2, PDLPOSTN, periostin, PN, POSTN, RP11 412K4.1

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Galerie der Validierungsdaten

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  IHC staining of human colon using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27794-1-AP (POSTN antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 27794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27794-1-AP (POSTN antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27794-1-AP bindet in IHC, ELISA Periostin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Periostin fusion protein Ag27145
• Vollständiger Name: periostin, osteoblast specific factor
• GenBank-Zugangsnummer: BC106710
• Gene symbol: POSTN
• Gene ID (NCBI): 10631
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance.