Palladin Polyklonaler Antikörper

Palladin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-10853

Synonyme

CGI 151, FLJ22190, FLJ38193, FLJ39139, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

---

Galerie der Validierungsdaten

• 

  IF Staining of mouse testis using CL555-10853

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 Palladin antibody (CL555-10853) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL555-10853

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 Palladin antibody (CL555-10853) at dilution of 1:200.

• 

  FC experiment of HeLa using CL555-10853

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Palladin (CL555-10853) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushodengewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-10853 bindet in IF, FC (Intra) Palladin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Palladin fusion protein Ag1288
• Vollständiger Name: palladin, cytoskeletal associated protein
• Berechnetes Molekulargewicht: 1383 aa, 151 kDa
• Beobachtetes Molekulargewicht: 95 kDa, 140 kDa
• GenBank-Zugangsnummer: BC013867
• Gene symbol: PALLD
• Gene ID (NCBI): 23022
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C