pregnancy zone protein Polyklonaler Antikörper

pregnancy zone protein Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 21742-1-AP

Synonyme

CPAMD6, pregnancy zone protein, PZP

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Galerie der Validierungsdaten

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  WB analysis using 21742-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21742-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, humanes Hirngewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21742-1-AP bindet in WB, IHC, ELISA pregnancy zone protein und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: pregnancy zone protein fusion protein Ag16471
• Vollständiger Name: pregnancy-zone protein
• Berechnetes Molekulargewicht: 1482 aa, 164 kDa
• Beobachtetes Molekulargewicht: 164 kDa
• GenBank-Zugangsnummer: BC111756
• Gene symbol: PZP
• Gene ID (NCBI): 5858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pregnancy Zone Protein (PZP) is an immunosuppressive protein that is expressed by the placenta. The PZP is a thiol ester-containing alpha macroglobulin protein that controls the proteolytic pathways during pregnancy and contributes to maintaining the immunologically privileged status of the fetus. Recently PZP over-expression has been described as a novel biomarker for predicting the prognosis of hepatocellular carcinoma and its high expression has been reported in solid tumors.

Publikationen

Species	Application	Title
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu