PYGM-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PYGM-Specific Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 19716-1-AP

Synonyme

Myophosphorylase, PYGM

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 19716-1-AP

  WB result of PYGM-Specific antibody (19716-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGM-Specific transfected HepG2 cells.

• 

  WB analysis using 19716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19716-1-AP (PYGM-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19716-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 19716-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19716-1-AP (PYGM-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, rat heart
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

19716-1-AP bindet in WB, IHC, IF, ELISA PYGM-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phosphorylase, glycogen, muscle
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97-105 kDa
• GenBank-Zugangsnummer: NM_005609
• Gene symbol: PYGM
• Gene ID (NCBI): 5837
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM.

Publikationen

Species	Application	Title
mouse	WB	Nature; Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.; Authors - Omer Keinan
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
mouse	WB	Dis Model Mech; Absence of p.R50X Pygm read-through in McArdle disease cellular models.; Authors - Guillermo Tarrasó
mouse	WB	Cells; Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein Interactome; Authors - Jia-Hua Qu
human	WB	Oncol Lett; Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.; Authors - Lingyu Jiang
human	WB	Clin Proteomics; The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma; Authors - Yang Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Patricia (Verified Customer) (02-25-2020)

Some non-specific bands appeared. Blocked in 5% skim milk and diluted antibody in TBS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer