PYCR1-specific Polyklonaler Antikörper

PYCR1-specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20962-1-AP

Synonyme

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

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Galerie der Validierungsdaten

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  WB analysis of Raji using 20962-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 20962-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20962-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 20962-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20962-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 20962-1-AP

  IP Result of anti-PYCR1 (IP:20962-1-AP, 4ug; Detection:20962-1-AP 1:500) with Raji cells lysate 1440ug.

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  IHC staining of human colon cancer using 20962-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20962-1-AP (PYCR1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 20962-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20962-1-AP (PYCR1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, humanes Hirngewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20962-1-AP bindet in WB, IP, IHC, ELISA PYCR1-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pyrroline-5-carboxylate reductase 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: NM_006907
• Gene symbol: PYCR1
• Gene ID (NCBI): 5831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody is specific to PYCR1.

Publikationen

Species	Application	Title
mouse	WB	EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human,mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell