Nectin-1/PVRL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Nectin-1/PVRL1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 24713-1-AP

Synonyme

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12

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Galerie der Validierungsdaten

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  WB analysis using 24713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24713-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 24713-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 24713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 24713-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe, SH-SY5Y-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

24713-1-AP bindet in WB, IHC, IF, ELISA Nectin-1/PVRL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nectin-1/PVRL1 fusion protein Ag20074
• Vollständiger Name: poliovirus receptor-related 1 (herpesvirus entry mediator C)
• Berechnetes Molekulargewicht: 517 aa, 57 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 57 kDa
• GenBank-Zugangsnummer: BC104948
• Gene symbol: PVRL1
• Gene ID (NCBI): 5818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).

Publikationen

Species	Application	Title
human	WB	PLoS Pathog; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.; Authors - Hiroki Takeuchi
human	IHC,WB	Front Oncol; High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma; Authors - Xuequan Wang ; KD Validated
mouse	IHC	Mol Ther Oncolytics; Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.; Authors - Gaku Takano
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
pig	WB,IF	J Virol; TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication; Authors - Xiu-Qing Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

This PVRL1 worked well with the ovarian cancer cell line OV8. It was more difficult to get a clear detection in the ovarian cancer cell line OV90, but we believe a similar trend was apparent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OV8 and 0V90

Nectin-1/PVRL1 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:24713-1-AP)