PTRF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PTRF Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (2)

Kat-Nr. : 18892-1-AP

Synonyme

Cavin 1, FKSG13, PTRF

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Galerie der Validierungsdaten

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  WB analysis using 18892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18892-1-AP (PTRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 18892-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18892-1-AP (PTRF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-1080 using 18892-1-AP

  IP result of anti-PTRF(IP:18892-1-AP, 4ug; Detection:18892-1-AP 1:2000) with HT-1080 cells lysate 3600 ug.

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  IHC staining of human pancreas using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18892-1-AP (PTRF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brown adipose using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 18892-1-AP (PTRF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 18892-1-AP (PTRF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18892-1-AP (PTRF antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 18892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (18892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, Mausherzgewebe, Mauslungengewebe, Rattenherzgewebe
• Erfolgreiche IP: HT-1080.Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, braunes Mausfettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• IP: See 2 publications below

Produktinformation

18892-1-AP bindet in WB, IP, IHC, IF, ELISA PTRF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTRF fusion protein Ag13486
• Vollständiger Name: polymerase I and transcript release factor
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC008849
• Gene symbol: PTRF
• Gene ID (NCBI): 284119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55kDa. (PMID: 11139612 )

Publikationen

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
human	WB,IF	Sci Adv; Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.; Authors - Virginia Andrade ; KO Validated
human	WB,IF,IP	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
human	WB,IHC	Autophagy; CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.; Authors - Xiangli Bai ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.; Authors - Eric Q Wei
mouse	IF	Theranostics; PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma.; Authors - Eryan Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Celine (Verified Customer) (06-22-2023)

Works very well in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Melanoma cell lines

PTRF Antibody Western Blot validation (1:1000 dilution) in Melanoma cell lines (Cat no:18892-1-AP)

FH

Maria (Verified Customer) (01-27-2022)

Great antibody. It worked perfectly for IF 1:200 in several cell types (RPE-1, adipocytes, mesenchymal, HeLa or MOVAS), for WB (1:2000) and for IP.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RPE-1 /MSC/MOVAS