PTPRJ Polyklonaler Antikörper

PTPRJ Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55123-1-AP

Synonyme

CD148, Density enhanced phosphatase 1, DEP 1, DEP1, HPTP eta, HPTPeta, PTPRJ, R PTP ETA, R PTP J, SCC1

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 55123-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 55123-1-AP

  WB result of 55123-1-AP.

• 

  WB analysis using 55123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55123-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 55123-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55123-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Multizellen/-gewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

55123-1-AP bindet in WB, IHC, ELISA PTPRJ und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein tyrosine phosphatase, receptor type, J
• Berechnetes Molekulargewicht: 146 kDa
• Beobachtetes Molekulargewicht: 146-170 kDa
• GenBank-Zugangsnummer: NM_002843
• Gene symbol: PTPRJ
• Gene ID (NCBI): 5795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction. The antibody is specific to PTPRJ.

Publikationen

Species	Application	Title
human	WB	J Pathol; The protein tyrosine phosphatase receptor type J is regulated by the pVHL-HIF axis in clear cell renal cell carcinoma.; Authors - Casagrande Silvia S
human	IHC	Onco Targets Ther; Comprehensive Analysis of Aberrantly Expressed Competitive Endogenous RNA Network and Identification of Prognostic Biomarkers in Pheochromocytoma and Paraganglioma.; Authors - Zijun Wang
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu