PTPRCAP / CD45AP Polyklonaler Antikörper

PTPRCAP / CD45AP Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19715-1-AP

Synonyme

CD45 AP, CD45 associated protein, LPAP, PTPRC associated protein, PTPRCAP, PTPRCAP / CD45AP

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 19715-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19715-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 19715-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 19715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPRCAP / CD45AP antibody (19715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Milzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19715-1-AP bindet in WB, IHC, IF, ELISA PTPRCAP / CD45AP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C-associated protein
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: NM_005608
• Gene symbol: PTPRCAP
• Gene ID (NCBI): 5790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRCAP, also named as LPAP and CD45-AP, interacts with CD45/PTPRC.